Journal: International Journal of Molecular Sciences

Article Title: Male and Female Animals Respond Differently to High-Fat Diet and Regular Exercise Training in a Mouse Model of Hyperlipidemia

doi: 10.3390/ijms22084198

Figure Lengend Snippet: Body weight and serum parameters. ( A ) Body weight and ( B ) serum glucose levels were measured at baseline and monthly thereafter during the experiment ( n = 10–12 animals/group). At the end of the experiment, serum samples were collected. ( C ) Serum TG levels were measured using an enzymatic colorimetric assay kit ( n = 10–12 animals/group). ( D ) Serum concentrations of leptin, ( E ) TNFα, and ( F ) resistin were quantified using a MILLIPLEX ® Mouse Adipokine Multiplex Immunoassay ( n = 10 animals/group). Values are presented as mean ± SEM; ○ indicates wild-type male group vs. wild-type female group; Δ indicates APOB-100 male group vs. APOB-100 female group; + indicates APOB-100 male group vs. wild type male group; # indicates APOB-100 female group vs. wild type female group; * denotes p < 0.05; ** denotes p < 0.01; *** denotes p < 0.001. WT/ND, wild-type mice on normal diet; APOB/HFD, APOB-100 transgenic mice on high-fat diet; TG, triglyceride.

Article Snippet: Luminex xMAP technology was used to determine the concentrations of 4 distinct proteins (leptin, resistin, insulin, TNF-α), by performing a MILLIPLEX ® Mouse Adipokine Multiplex Immunoassay (MADKMAG-71K, Merck Millipore, Darmstadt, Germany) according to the instructions of the manufacturer.

Techniques: Enzymatic Colorimetric Assay, Multiplex Assay, Transgenic Assay